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| Registros recuperados: 69 | |
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| Horn, Karin; Ono, Kenichiro; Heydorn, Alfred Otto. |
| This paper describes a standard method for in vitro excystation of sporocysts of S.capracanis, S.hircicanis, S.ovicanis and S.arieticanis. A standard pretreatment was performed by 20 minutes preincubation of sporocysts in aqueous sodium hypochlorite (NaOCl) solution of 6% or 8% at room temperature. Then, sporocysts were washed 5 times in distilled water and were incubated for one hour at 39OC in excystation fluid consisting of RPMI 1640 medium, 10% PCS and 15% bovine bile. Additional sonication of pretreated sporocysts increased excystation rates as compared to not sonicated controls. Excystation rates for 1-9 months old sporocysts were 77% for S.capracanis, 77% for S.hircicanis, 72% for S.arieticanis and 92% for S.ovicanis. Sporozoite suspension as... |
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Palavras-chave: Cryopreservation; In vitro excystation; Sarcocystis spp.; Sporozoites. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/154 |
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| Suquet, Marc; Dreanno, Catherine; Petton, Bruno; Normant, Yvon; Omnes, Marie-helene; Billard, Roland. |
| The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use... |
| Tipo: Text |
Palavras-chave: Turbot; Psetta maxima; Sperm; Cryopreservation; Hatching. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00176/28721/27176.pdf |
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| Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno. |
| A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied.... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf |
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| Labruna,M.B.; Resende,J.S.; Martins,N.R.S.; Jorge,M.A.. |
| Soros de aves experimentalmente infectadas, contendo espiroquetas viáveis, foram submetidos a dois procedimentos antes da criopreservação: glicerol na diluição de 1/2 (v/v), designado como soro com glicerol a 50% (GS), e dimetilsulfóxido na proporção de 1/10 (v/v), designado como soro com DMSO a 10% (DS). Após 15 meses de estocagem em nitrogênio líquido, amostras dos tratamentos GS e DS foram descongeladas e suas infectividades foram testadas em frangos susceptíveis. Apesar de ambos os procedimentos terem mantidos a infectividade da bactéria, DMSO a 10% no soro de frango apresentou-se mais satisfatório como criopreservante. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Chicken; Spirochete; Borrelia anserina; Cryopreservation. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000600008 |
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| Suquet, Marc; Dreanno, Catherine; Fauvel, Christian; Cosson, J; Billard, R. |
| Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results.... |
| Tipo: Text |
Palavras-chave: DMSO; Insemination; Fertilization; Cryopreservation; Fish sperm. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-611.pdf |
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| SEIJO,GUILLERMO. |
| Changes in cell water relations during precultures were followed in an attempt to understand the mechanism of cell hardening for cryopreservation by vitrification. Medium containing 0.4 M sucrose (psiw=-1.45 MPa) and containing 5 mg L-1 of ABA (MT psiw=-0.73 MPa and MS psiw=-0.48 MPa) were used to harden cell suspensions of orange and carrot. Preculture in these medium did not cause a significant decrease of cell viability, however, it improved the cell survival to PVS2 and liquid nitrogen expositions. When cells were inoculated into medium containing 0.4 M sucrose, the cell psiw decreased rapidly until reach the medium psiw , and turgor was also severely reduced or disappeared. Subsequently, cell psis began to decrease and after 48 h of treatment in low... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preculture; Cell suspension; Sucrose; ABA; Cryopreservation. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312000000200007 |
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| STEINER,A. A.; PETENUSCI,S. O.; BRENTEGANI,L. G.; BRANCO,L. G. S.. |
| Several species of terrestrially hibernating frogs, turtles and insects have developed mechanisms, such as increased plasma glucose, anti-freeze proteins and antioxidant enzymes that resist to freezing, for survival at subzero temperatures. In the present study, we assessed the importance of glucose to cryoresistance of two anuran amphibians: the frog Rana catesbeiana and the toad Bufo paracnemis. Both animals were exposed to -2ºC for measurements of plasma glucose levels, liver and muscle glycogen content, haematocrit and red blood cell volume. Frogs survived cold exposure but toads did not. Blood glucose concentration increased from 40.35 ± 7.25 to 131.87 ± 20.72 mg/dl (P < 0.01) when the frogs were transferred from 20 to -2ºC. Glucose accumulation in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bufo paracnemis; Rana catesbeiana; Freeze tolerance; Cryopreservation; Glucose. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200017 |
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| Salomão,Antonieta Nassif. |
| The ability to tolerate ultra-low temperature (-196 ºC) exposure was evaluated in 66 tropical orthodox seed species of 21 botanical families from the Cerrado (Brazilian Savannah) and Atlantic Forest Brazilian biomes. Liquid nitrogen had no effect on the germinability of 51 seed species. The stimulatory effect of cryogenic temperature on germinability, with or without subsequent chemical scarification, was observed in nine seed species with deep physical dormancy, or heterogeneous levels of seed hardness, or with no dormancy. Significant reduction in germinability occurred in six seed species, presumably because of factors acting individually or in combination on these seeds, such as inappropriate moisture content, or the potentially detrimental effect of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryopreservation; Ex situ conservation; Seed dormancy. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000200008 |
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| Chatain, Beatrice; Poline, Olivier. |
| Objectives : Presently, most of marine fish farmers around the Mediterranean basin grow genetically wild fish strains, which have not been genetically improved for culture performance (growth, appearance, disease resistance etc.). It is inevitable that the present gap for genetically improved strains of marine fish, and their reliable supply, will be filled. It will ultimately dominate the competitive component of marine culture industries once they evolve into commodity-style agribusiness (with fish prices being set at the lowest possible cost of production and the best quality). Those countries, which can competitively develop and supply the improved strains, will enjoy the benefit (perhaps survival) for their local industries. They will avoid... |
| Tipo: Text |
Palavras-chave: Pathogen free; Genetic origin; Sperm; Cryopreservation; Sea bass. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/rapport-6494.pdf |
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| Hanquet, Anne-caroline; Kellner, K; Heude, C; Naimi, Amine; Mathieu, M; Poncet, J.m.. |
| Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me2SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees C min(-1) down to -70 degrees C before transfer into... |
| Tipo: Text |
Palavras-chave: Glycogen metabolism; Vesicular cells; Cryopreservation; Mollusc; Oyster; Crassostrea gigas. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1938.pdf |
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| Oliveira,E.C.S.; Juliani,G.C.; Marques Jr.,A.P.; Henry,M.. |
| The efficacy of three extenders, tris-egg yolk-5% ethylene glycol (T1), lactose-egg yolk-5% ethylene glycol (T2) and lactose-egg yolk-5% dimethyl formamide (T3) on preserving the viability of post-thawing canine spermatozoa was evaluated. Three ejaculates per dog were obtained of five animals. The semen was packaged in 0.5ml straws and cooled to 4°C for 120min. The straws were frozen 4cm above the nitrogen level for 15min and thawed in water-bath at 37°C for 60sec and at 75°C for 7sec. Progressive motility and vigour were evaluated immediately after thawing (time 0) and at 30, 60, 90 and 120min. Structural and functional integrity of plasma membrane of the spermatozoa were evaluated, respectively, by fluorescent staining probes and hypoosmotic swelling... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Semen; Cryopreservation; Ethylene glycol; Dimethyl formamide. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600021 |
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| Ninhaus-Silveira,Alexandre; Foresti,Fausto; Veríssimo-Silveira,Rosicleire; Senhorini,José Augusto. |
| Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 x 10(6) spermatozoa/mm³. Spermatozoa (length = 31.288 ±4.47µm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 µm; width = 1.752 ±0.17µm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561±0.44µm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37µm). Fertilization tests with thawed semen demonstrated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Semen characteristics; Cryopreservation; Brycon cephalus; Ultrastructure; Fish. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500015 |
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| Registros recuperados: 69 | |
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